1. Funding period












A 01 Erhard Bremer

  1. Widderich N, Höppner A, Pittelkow M, Heider J, Smits, Sander H J, Bremer E. 2014. Biochemical properties of ectoine hydroxylases from extremophiles and their wider taxonomic distribution among microorganisms. PLoS ONE 9(4):e93809. doi:10.1371/journal.pone.0093809.
  2. Widderich N, Pittelkow M, Höppner A, Mulnaes D, Buckel W, Gohlke H, Smits, Sander H J, Bremer E. 2014. Molecular dynamics simulations and structure-guided mutagenesis provide insight into the architecture of the catalytic core of the ectoine hydroxylase. J Mol Biol 426(3):586–600. doi:10.1016/j.jmb.2013.10.028.
  3. Becker J, Schäfer R, Kohlstedt M, Harder BJ, Borchert NS, Stöveken N, Bremer E, Wittmann C. 2013. Systems metabolic engineering of Corynebacterium glutamicum for production of the chemical chaperone ectoine. Microb Cell Fact 12:110. doi:10.1186/1475-2859-12-110.
  4. Bremer E. 2014. Liberate and grab it, ingest and digest it: the GbdR regulon of the pathogen Pseudomonas aeruginosa. J Bacteriol 196(1):3–6. doi:10.1128/JB.01243-13.
  5. Hoeppner A, Widderich N, Bremer E, Smits, Sander H J. 2014. Overexpression, crystallization and preliminary X-ray crystallographic analysis of the ectoine hydroxylase from Sphingopyxis alaskensis. Acta Crystallogr F Struct Biol Commun 70(Pt 4):493–496. doi:10.1107/S2053230X14004798.
  6. Höppner A, Widderich N, Lenders M, Bremer E, Smits, Sander H J. 2014. Crystal structure of the ectoine hydroxylase, a snapshot of the active site. J Biol Chem 289(43):29570–29583. doi:10.1074/jbc.M114.576769.
  7. Kobus S, Widderich N, Hoeppner A, Bremer E, Smits, Sander H J. 2015. Overproduction, crystallization and X-ray diffraction data analysis of ectoine synthase from the cold-adapted marine bacterium Sphingopyxis alaskensis. Acta Crystallogr F Struct Biol Commun 71(Pt 8):1027–1032. doi:10.1107/S2053230X15011115.
  8. Widderich N, Czech L, Elling FJ, Könneke M, Stöveken N, Pittelkow M, Riclea R, Dickschat JS, Heider J, Bremer E. 2016. Strangers in the archaeal world: osmostress-responsive biosynthesis of ectoine and hydroxyectoine by the marine thaumarchaeon Nitrosopumilus maritimus. Environ Microbiol. 18:1227-1248. doi: 10.1111/1462-2920.13156.
  9. Widderich N, Kobus S, Höppner A, Riclea R, Seubert A, Dickschat JS, Heider J, Smits SHJ, Bremer E. 2016. Biochemistry and crystal structure of ectoine synthase: a metal-containing member of the cupin superfamily. PLoS ONE 11(3):e0151285. doi:10.1371/journal.pone.0151285.
  10. Schulz A, Stöveken N, Binzen I, Hoffmann T, Heider J, Bremer E. 2016. Feeding on compatible solutes: a substrate-induced pathway for uptake and catabolism of ectoines and its genetic control by EnuR. Env. Microbiol. doi: 10.1111/1462-2920.13414.
    Widderich N, Bremer E, Smits SHJ. (2016) The ectoine hydroxylase: a non-heme-containing iron (II) and 2-oxoglutarate-dependent dioxygenase. Encyclopedia of Inorganic and Bioinorganic Chemistry. 1-10. (Ed. A. Messerschmidt).